Match the stationary phase (Column I) with its corresponding chromatography
technique (Column II).
Column I
Column II
P. Protein A
1. Size exclusion chromatography
Q. Sephadex
2. Ion-exchange chromatography
R. Phenylsepharose
3. Affinity chromatography
S. Diethylaminoethyl cellulose
4. Hydrophobic interaction chromatography
Options:
(A) P-1, Q-4, R-2, S-3
(B) P-3, Q-1, R-4, S-2
(C) P-3, Q-4, R-2, S-1
(D) P-4, Q-1, R-3, S-2
Official Answer Key:
B
Step-by-Step Solution & Explanation
### **Official Solution & Concept Analysis (GATE BT 2022 Q.40)**
**1. Governing Concept**:
- **Topic**: Downstream Processing & Chromatography Matrices
- Matching chromatographic stationary phases with separation techniques:
- **P. Protein A** $\rightarrow$ Bacterial cell wall protein binding Fc region of IgG antibodies $\rightarrow$ **Affinity chromatography** (3)
- **Q. Sephadex** $\rightarrow$ Cross-linked dextran porous gel beads separating molecules by hydrodynamic volume $\rightarrow$ **Size exclusion chromatography** (1)
- **R. Phenylsepharose** $\rightarrow$ Agarose matrix derivatized with hydrophobic phenyl groups $\rightarrow$ **Hydrophobic interaction chromatography (HIC)** (4)
- **S. Diethylaminoethyl (DEAE) cellulose** $\rightarrow$ Positively charged tertiary amine resin acting as a weak anion exchanger $\rightarrow$ **Ion-exchange chromatography** (2)
**2. Option Analysis**:
- **Option (A) is INCORRECT**: Misassigns Protein A to size exclusion.
- **Option (B) [P-3, Q-1, R-4, S-2] is CORRECT**: Every chromatographic stationary phase is accurately paired with its operational principle.
- **Option (C) is INCORRECT**: Misassigns Phenylsepharose to ion exchange.
- **Option (D) is INCORRECT**: Misassigns DEAE cellulose to affinity.
**3. Final Answer**:
- **Correct Choice**: **B**
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