Section 4: Bioprocess Engineering and Process Biotechnology
Bioreactor types, scale-up
Subtopic: Fundamentals of Bioreactor types, scale-up
Question Statement:
Match the media component used in mammalian cell culture (Column I) with its
respective role (Column II).
Column I
Column II
P. Hydrocortisone
1. Mitogen
Q. Fibronectin
2. Vitamin
R. Epidermal growth factor
3. Hormone
S. Riboflavin
4. Cell attachment
Options:
(A) P-3, Q-4, R-1, S-2
(B) P-3, Q-4, R-2, S-1
(C) P-4, Q-3, R-1, S-2
(D) P-4, Q-3, R-2, S-1
Official Answer Key:
A
Step-by-Step Solution & Explanation
### **Official Solution & Concept Analysis (GATE BT 2022 Q.37)**
**1. Governing Concept**:
- **Topic**: Animal Cell Culture Media Formulation
- Specific components in mammalian cell culture media:
- **Fetal Bovine Serum (FBS)**: Provides growth factors, hormones, attachment factors, and binding proteins.
- **Sodium bicarbonate ($\text{NaHCO}_3$)**: Acts as a chemical buffer in equilibrium with dissolved $\text{CO}_2$ ($5\% \text{ CO}_2$ incubator) to maintain physiological pH ($7.2-7.4$).
- **L-Glutamine**: Unstable essential amino acid providing nitrogen and energy source.
- **Pluronic F-68 (poloxamer 188)**: Non-ionic surfactant added to protect shear-sensitive animal cells from hydrodynamic shear stress and bubble bursting damage in agitated/sparged bioreactors.
**2. Option Analysis**:
- **Option (A) is CORRECT**: Accurately matches each mammalian media component with its specific physiological function.
- **Option (B) is INCORRECT**: Misassigns Pluronic F-68 to buffering.
- **Option (C) is INCORRECT**: Inverts serum and glutamine roles.
- **Option (D) is INCORRECT**: Misidentifies shear-protecting surfactants.
**3. Final Answer**:
- **Correct Choice**: **A**
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