GATE BT 2022 • Q26 MCQ • 1 Mark

Section 3: Genetics, Cellular and Molecular Biology

Recombinant DNA technology

Subtopic: Fundamentals of Recombinant DNA technology

Question Statement:

Among individuals in a human population, minor variations exist in nucleotide sequences of chromosomes. These variations can lead to gain or loss of sites for specific restriction enzymes. Which of the following technique is used to identify such variations?

Options:

  • (A) Polymerase dependent fragment insertion
  • (B) Real-time polymerase chain reaction
  • (C) Restriction fragment length polymorphism
  • (D) Reverse transcriptase polymerase chain reaction

Official Answer Key:

C

Step-by-Step Solution & Explanation

### **Official Solution & Concept Analysis (GATE BT 2022 Q.26)** **1. Governing Concept**: - **Topic**: Human Genetics & Genomic Variations - Among individuals in human populations: - Single nucleotide differences present in
gt; 1\%$ of the population are termed **Single Nucleotide Polymorphisms (SNPs)**. - SNPs represent the most abundant type of genetic variation in the human genome ($\sim 4-5$ million SNPs per individual genome) and serve as primary genetic markers in GWAS, forensics, and personalized medicine. **2. Option Analysis**: - **Option (A) is INCORRECT**: Large chromosomal translocations are rare structural rearrangements. - **Option (B) is INCORRECT**: Aneuploidies are pathological chromosomal numerical abnormalities. - **Option (C) [Single Nucleotide Polymorphisms] is CORRECT**: The predominant form of minor inter-individual genetic variation. - **Option (D) is INCORRECT**: Inversions are less frequent than single base changes. **3. Final Answer**: - **Correct Choice**: **C**

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