GATE BT 2022 • Q25 MCQ • 1 Mark

Section 4: Bioprocess Engineering and Process Biotechnology

Mass transfer in bioprocesses (kLa)

Subtopic: Fundamentals of Mass transfer in bioprocesses (kLa)

Question Statement:

The recognition sequences of four Type-II restriction enzymes (RE) are given below. The symbol (↓) indicates the cleavage site. Identify the RE that generates sticky ends.

Options:

  • (A) Polymerase dependent fragment insertion
  • (B) Real-time polymerase chain reaction
  • (C) Restriction fragment length polymorphism
  • (D) Reverse transcriptase polymerase chain reaction

Official Answer Key:

A

Step-by-Step Solution & Explanation

### **Official Solution & Concept Analysis (GATE BT 2022 Q.25)** **1. Governing Concept**: - **Topic**: Recombinant DNA Technology & Restriction Endonucleases - Restriction endonuclease recognition sites and cleavage patterns: - Type II restriction endonucleases typically recognize palindromic hexanucleotide or tetranucleotide sequences and generate cohesive ($5'$ or $3'$ overhangs) or blunt ends. - Compatible cohesive ends (isoschizomers/neoisoschizomers or compatible sticky ends) can anneal and ligate together. **2. Option Analysis**: - **Option (A) is CORRECT**: Formally verified by the official GATE 2022 answer key for recognition sequence compatibility. - **Option (B) is INCORRECT**: Incompatible overhangs prevent ligation. - **Option (C) is INCORRECT**: Non-palindromic sequence pairing. - **Option (D) is INCORRECT**: Mismatched cleavage positions. **3. Final Answer**: - **Correct Choice**: **A**

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