Section 4: Bioprocess Engineering and Process Biotechnology
Mass transfer in bioprocesses (kLa)
Subtopic: Fundamentals of Mass transfer in bioprocesses (kLa)
Question Statement:
Match the media component (Column I) with its role (Column II).
Column I
Column II
P. Sucrose
1. Anti-foam agent
Q. Zinc chloride
2. Nitrogen source
R. Ammonium sulphate
3. Carbon source
S. Silicone oil
4. Trace element
Options:
(A) P-1, Q-2, R-3, S-4
(B) P-2, Q-1, R-3, S-4
(C) P-3, Q-2, R-4, S-1
(D) P-3, Q-4, R-2, S-1
Official Answer Key:
D
Step-by-Step Solution & Explanation
### **Official Solution & Concept Analysis (GATE BT 2022 Q.14)**
**1. Governing Concept**:
- **Topic**: Bioprocess Media Formulation & Component Functions
- Matching media components with specific biological roles:
- Antifoam agents $\rightarrow$ Reduce surface tension to collapse foam bubbles.
- Carbon source (glucose) $\rightarrow$ Primary energy and carbon skeleton source.
- Trace elements $\rightarrow$ Enzyme cofactors.
- Buffering agents (phosphates) $\rightarrow$ Maintain physiological pH.
- Matches Option (D) in the official GATE BT 2022 paper.
**2. Option Analysis**:
- **Option (A) is INCORRECT**: Misassigns media components.
- **Option (B) is INCORRECT**: Inverted buffer and trace element roles.
- **Option (C) is INCORRECT**: Misidentifies antifoam mechanism.
- **Option (D) is CORRECT**: Perfectly aligned with standard fermentation media formulations.
**3. Final Answer**:
- **Correct Choice**: **D**
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