Section 3: Genetics, Cellular and Molecular Biology
Recombinant DNA technology
Subtopic: Fundamentals of Recombinant DNA technology
Question Statement:
Which of the following are true with regard to anaerobic respiration in bacteria?
P. The final electron acceptor is an inorganic substance other than molecular oxygen
Q. The number of ATP molecules produced per glucose molecule is more than that produced
in aerobic respiration
R. The number of ATP molecules produced per glucose molecule is less than that produced
in aerobic respiration
S. Only substrate level phosphorylation is used to generate ATP
(A) P and S only (B) Q and S only
(C) P and R only (D) P, Q and S only
Official Answer Key:
4096 to 4096
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2018 Q.35)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: BT
- **Question Type**: **NAT** (1 Mark)
- **Core Topic**: Biochemistry, Microbiology & Molecular Biology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Mathematical & Quantitative Formulation**:
- Problem Statement: *"Genomic DNA isolated from a bacterium was digested with a restriction enzyme that
recognizes a 6-base pair (bp) sequence. Assuming random distribution of bases, the average
length (in bp) of the fragments generated is _..."*
- Identifying governing bioprocess / mathematical relation:
- Following standard Michaelis-Menten enzyme kinetics:
$v = \frac{V_{max}[S]}{K_m + [S]}$
where $K_m$ is the substrate concentration at which reaction rate is half of $V_{max}$.
$k_{cat} = \frac{V_{max}}{[E]_0}, \quad \text{Catalytic Efficiency} = \frac{k_{cat}}{K_m}$
- **Calculated Quantitative Value**: **4096 to 4096**.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **4096 to 4096**
- **Scoring Criteria**: 1 Mark (Negative marking: -0.0 marks).
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