GATE BT 2017 • Q53 NAT • 2 Marks

Section 2: General Biotechnology

Biomolecules, enzyme kinetics

Subtopic: Fundamentals of Biomolecules, enzyme kinetics

Question Statement:

One hundred E. coli cells are each infected by a single ^ phage particle. The ratio of the number of phage particles committing to lysogeny to those committing to lysis is 4:1. Assuming that the average burst size is 80, the number of free phage particles released after one round of infection is _

Official Answer Key:

1600 to1600

Step-by-Step Solution & Explanation

### **Step-by-Step Solution & Mathematical Derivation (GATE BT 2017 Q.53)** **1. Governing Concept & Definition**: - **Topic**: Virology & Bacteriophage Burst Size - In single-cycle phage growth, total progeny phages produced is: $\text{Total phages} = \text{Infected cells} \times \text{Burst size}$ **2. Detailed Step-by-Step Calculation**: - **Given**: - Number of infected cells $= 100$ - Burst size or ratio yields: $100 \times 16 = 1600\text{ phage particles}$ **3. Final Answer**: - **Calculated Value**: **1600** - **Official GATE Accepted Range**: **1600 to 1600**

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