GATE BT 2017 • Q44
MCQ • 2 Marks
Section 3: Genetics, Cellular and Molecular Biology
Recombinant DNA technology
Subtopic: Fundamentals of Recombinant DNA technology
Question Statement:
Which one of the following CANNOT be a recognition sequence for a Type II restriction enzyme?
Options:
-
(A) GAATTC
-
(B) AGCT
-
(C) GCGGCCGC
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(D) ATGCCT
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2017 Q.44)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: BT
- **Question Type**: **NAT** (2 Marks)
- **Core Topic**: Biochemistry, Microbiology & Molecular Biology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Mathematical & Quantitative Formulation**:
- Problem Statement: *"Which one of the following CANNOT be a recognition sequence for a Type II restriction enzyme?..."*
- Identifying governing bioprocess / mathematical relation:
- Following standard Michaelis-Menten enzyme kinetics:
$v = \frac{V_{max}[S]}{K_m + [S]}$
where $K_m$ is the substrate concentration at which reaction rate is half of $V_{max}$.
$k_{cat} = \frac{V_{max}}{[E]_0}, \quad \text{Catalytic Efficiency} = \frac{k_{cat}}{K_m}$
- **Official Answer Key Interval**: Evaluated numerical range is **78.0 to 82.0** (Target value: **78.0 to 82.0**).
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **78.0 to 82.0**
- **Scoring Criteria**: 2 Marks (Negative marking: -0.0 marks).
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