Subtopic: Fundamentals of Biomolecules, enzyme kinetics
Question Statement:
The yield (%) for each step is
(A) P-10, Q-7.2, R-4.5, S-2.0 (B) P-34, Q-24, R-15, S-1
(C) P-3.4, Q-2.4, R-1.5, S-0.1 (D) P-100, Q-71, R-44, S-29
Linked Answer Questions
Statement for Linked Answer Questions 52 and 53:
An E. coli cell of volume 10 -12 cm3 contains 60 molecules of lac -repressor. The repressor has a binding
affinity (Kd) of 10-8 M and 10-9 M with and without lactose respectively, in the medium.
Options:
(A) P-10, Q-7.2, R-4.5, S-2.0
(B) P-34, Q-24, R-15, S-1
(C) P-3.4, Q-2.4, R-1.5, S-0.1
(D) P-100, Q-71, R-44, S-29
Linked Answer Questions
Statement for Linked Answer Questions 52 and 53:
An E. coli cell of volume 10 -12 cm3 contains 60 molecules of lac -repressor. The repressor has a binding
affinity (Kd) of 10-8 M and 10-9 M with and without lactose respectively, in the medium.
Official Answer Key:
D
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2012 Q.51)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: BT
- **Question Type**: **MCQ** (2 Marks)
- **Core Topic**: Biochemistry, Microbiology & Molecular Biology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"The yield (%) for each step is..."*
- **Option (D) [CORRECT]**: *"P-100, Q-71, R-44, S-29
Linked Answer Questions
Statement for Linked Answer Questions 52 and 53:
An E. coli cell of volume 10 -12 cm3 contains 60 molecules of lac -repressor. The repressor has a binding
affinity (Kd) of 10-8 M and 10-9 M with and without lactose respectively, in the medium."*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (A) [INCORRECT]**: *"P-10, Q-7.2, R-4.5, S-2.0"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (B) [INCORRECT]**: *"P-34, Q-24, R-15, S-1"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (C) [INCORRECT]**: *"P-3.4, Q-2.4, R-1.5, S-0.1"* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **D**
- **Scoring Criteria**: 2 Marks (Negative marking: -0.66 marks).
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