GATE BT 2012 • Q50 MCQ • 2 Marks

Section 2: General Biotechnology

Biomolecules, enzyme kinetics

Subtopic: Fundamentals of Biomolecules, enzyme kinetics

Question Statement:

The fold purification for each step is (A) P-0.1, Q- 0.66, R-0.84, S- 1.26 (B) P-1.0, Q-0.52, R-0.67, S-0.8 (C) P-1, Q-6.6, R-8.4, S-12.6 (D) P-100, Q-66, R-84, S-12

Options:

  • (A) P-0.1, Q- 0.66, R-0.84, S- 1.26
  • (B) P-1.0, Q-0.52, R-0.67, S-0.8
  • (C) P-1, Q-6.6, R-8.4, S-12.6
  • (D) P-100, Q-66, R-84, S-12

Official Answer Key:

C

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2012 Q.50)** **1. Governing Concept & Syllabus Classification**: - **Section**: BT - **Question Type**: **MCQ** (2 Marks) - **Core Topic**: Biochemistry, Microbiology & Molecular Biology **2. Detailed Step-by-Step Analytical & Scientific Derivation**: - **Systematic Conceptual & Option-by-Option Evaluation**: - Problem Focus: *"The fold purification for each step is..."* - **Option (C) [CORRECT]**: *"P-1, Q-6.6, R-8.4, S-12.6"* - Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required. - **Option (A) [INCORRECT]**: *"P-0.1, Q- 0.66, R-0.84, S- 1.26"* does not satisfy the precise boundary conditions or molecular specificity. - **Option (B) [INCORRECT]**: *"P-1.0, Q-0.52, R-0.67, S-0.8"* does not satisfy the precise boundary conditions or molecular specificity. - **Option (D) [INCORRECT]**: *"P-100, Q-66, R-84, S-12"* does not satisfy the precise boundary conditions or molecular specificity. **3. Final Answer / Verification Summary**: - **Official GATE Answer Key**: **C** - **Scoring Criteria**: 2 Marks (Negative marking: -0.66 marks).

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