Section 4: Bioprocess Engineering and Process Biotechnology
Bioreactor types, scale-up
Subtopic: Fundamentals of Bioreactor types, scale-up
Question Statement:
Match the entries in Group I with the methods of sterilization in Group II.
Group I Group II
P. Serum 1. Autoclave
Q. Luria broth 2. Membrane filtration
R. Polypropylene tubes 3. UV irradiation
S. Biological safety cabinets 4. Gamma irradiation
5. Dry heat
(A) P-5, Q-3, R-1, S-4 (B) P-1, Q-4, R-5, S-3
(C) P-2, Q-1, R-4, S-3 (D) P-4, Q-1, R-3, S-5
Options:
(A) P-5, Q-3, R-1, S-4
(B) P-1, Q-4, R-5, S-3
(C) P-2, Q-1, R-4, S-3
(D) P-4, Q-1, R-3, S-5
Official Answer Key:
C
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2012 Q.42)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: BT
- **Question Type**: **MCQ** (2 Marks)
- **Core Topic**: Bioprocess Engineering
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"Match the entries in Group I with the methods of sterilization in Group II.
Group I Group II
P. Serum 1. Autoclave
Q. Luria broth 2. Membrane filtration
R. Polypropylene tubes 3. UV irradiation
S. Biological saf..."*
- **Option (C) [CORRECT]**: *"P-2, Q-1, R-4, S-3"*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (A) [INCORRECT]**: *"P-5, Q-3, R-1, S-4"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (B) [INCORRECT]**: *"P-1, Q-4, R-5, S-3"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (D) [INCORRECT]**: *"P-4, Q-1, R-3, S-5"* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **C**
- **Scoring Criteria**: 2 Marks (Negative marking: -0.66 marks).
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