GATE BT 2012 • Q27 MCQ • 2 Marks

Section 2: General Biotechnology

Biomolecules, enzyme kinetics

Subtopic: Fundamentals of Biomolecules, enzyme kinetics

Question Statement:

In zinc finger proteins, the amino acid residues that coordinate zinc are (A) Cys and His (B) Asp and Glu (C) Arg and Lys (D) Asp and Arg

Options:

  • (A) Cys and His
  • (B) Asp and Glu
  • (C) Arg and Lys
  • (D) Asp and Arg

Official Answer Key:

A

Step-by-Step Solution & Explanation

### **Official Solution & Step-by-Step Derivation (GATE BT 2012 Q.27)** **1. Governing Concept & Syllabus Classification**: - **Section**: BT - **Question Type**: **MCQ** (2 Marks) - **Core Topic**: Biochemistry, Microbiology & Molecular Biology **2. Detailed Step-by-Step Analytical & Scientific Derivation**: - **Systematic Conceptual & Option-by-Option Evaluation**: - Problem Focus: *"In zinc finger proteins, the amino acid residues that coordinate zinc are..."* - **Option (A) [CORRECT]**: *"Cys and His"* - Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required. - **Option (B) [INCORRECT]**: *"Asp and Glu"* does not satisfy the precise boundary conditions or molecular specificity. - **Option (C) [INCORRECT]**: *"Arg and Lys"* does not satisfy the precise boundary conditions or molecular specificity. - **Option (D) [INCORRECT]**: *"Asp and Arg"* does not satisfy the precise boundary conditions or molecular specificity. **3. Final Answer / Verification Summary**: - **Official GATE Answer Key**: **A** - **Scoring Criteria**: 2 Marks (Negative marking: -0.66 marks).

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