Subtopic: Fundamentals of Biomolecules, enzyme kinetics
Question Statement:
The helix content of a protein can be determined using
(A) an infrared spectrometer (B) a fluorescence spectrometer
(C) a circular dichroism spectrometer (D) a UV-Visible spectrophotometer
Q. 26 to Q. 55 carry two marks each.
Options:
(A) an infrared spectrometer
(B) a fluorescence spectrometer
(C) a circular dichroism spectrometer
(D) a UV-Visible spectrophotometer
Q. 26 to Q. 55 carry two marks each.
Official Answer Key:
C
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2012 Q.25)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: BT
- **Question Type**: **MCQ** (1 Mark)
- **Core Topic**: Biochemistry, Microbiology & Molecular Biology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"The helix content of a protein can be determined using..."*
- **Option (C) [CORRECT]**: *"a circular dichroism spectrometer"*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (A) [INCORRECT]**: *"an infrared spectrometer"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (B) [INCORRECT]**: *"a fluorescence spectrometer"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (D) [INCORRECT]**: *"a UV-Visible spectrophotometer
Q. 26 to Q. 55 carry two marks each."* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **C**
- **Scoring Criteria**: 1 Mark (Negative marking: -0.33 marks).
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