Subtopic: Fundamentals of Biomolecules, enzyme kinetics
Question Statement:
In transmission electron microscopy, electron opacity is greatly enhanced by treating the specimen
with
(A) ferrous ammonium sulfate (B) uranium acetate
(C) sodium chloride (D) basic fuchsin
Options:
(A) ferrous ammonium sulfate
(B) uranium acetate
(C) sodium chloride
(D) basic fuchsin
Official Answer Key:
B
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2012 Q.23)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: BT
- **Question Type**: **MCQ** (1 Mark)
- **Core Topic**: Biochemistry, Microbiology & Molecular Biology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"In transmission electron microscopy, electron opacity is greatly enhanced by treating the specimen
with..."*
- **Option (B) [CORRECT]**: *"uranium acetate"*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (A) [INCORRECT]**: *"ferrous ammonium sulfate"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (C) [INCORRECT]**: *"sodium chloride"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (D) [INCORRECT]**: *"basic fuchsin"* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **B**
- **Scoring Criteria**: 1 Mark (Negative marking: -0.33 marks).
Practice This in Real CBT Exam Interface
Test yourself with the official TCS-iON palette, virtual calculator, and immediate performance analytics.