GATE BT 2012 • Q2
MCQ • 1 Mark
Section 2: General Biotechnology
Biomolecules, enzyme kinetics
Subtopic: Fundamentals of Biomolecules, enzyme kinetics
Question Statement:
The basis for blue-white screening with pUC vectors is
(A) intraallelic complementation (B) intergenic complementation
(C) intragenic suppression (D) extragenic suppression
Options:
-
(A) intraallelic complementation
-
(B) intergenic complementation
-
(C) intragenic suppression
-
(D) extragenic suppression
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2012 Q.2)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: BT
- **Question Type**: **MCQ** (1 Mark)
- **Core Topic**: Quantitative & Analytical Aptitude
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"The basis for blue-white screening with pUC vectors is..."*
- **Option (A) [CORRECT]**: *"intraallelic complementation"*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (B) [INCORRECT]**: *"intergenic complementation"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (C) [INCORRECT]**: *"intragenic suppression"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (D) [INCORRECT]**: *"extragenic suppression"* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **A**
- **Scoring Criteria**: 1 Mark (Negative marking: -0.33 marks).
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