Subtopic: Fundamentals of Biomolecules, enzyme kinetics
Question Statement:
Which one of the following is NOT a protoplast fusion inducing agent?
(A) Inactivated Sendai virus (B) Ca2+ at alkaline pH
(C) Polyethylene glycol (D) Colchicine
Options:
(A) Inactivated Sendai virus
(B) Ca2+ at alkaline pH
(C) Polyethylene glycol
(D) Colchicine
Official Answer Key:
D
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2012 Q.16)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: BT
- **Question Type**: **MCQ** (1 Mark)
- **Core Topic**: Biochemistry, Microbiology & Molecular Biology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"Which one of the following is NOT a protoplast fusion inducing agent?..."*
- **Option (D) [CORRECT]**: *"Colchicine"*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (A) [INCORRECT]**: *"Inactivated Sendai virus"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (B) [INCORRECT]**: *"Ca2+ at alkaline pH"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (C) [INCORRECT]**: *"Polyethylene glycol"* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **D**
- **Scoring Criteria**: 1 Mark (Negative marking: -0.33 marks).
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