Subtopic: Fundamentals of Downstream processing (chromatography, filtration)
Question Statement:
Protein-DNA interactions in vivo can be studied by
(A) gel shift assay (B) Southern hybridization
(C) chromatin immunoprecipitation assay (D) fluorescence in situ hybridization assay
Options:
(A) gel shift assay
(B) Southern hybridization
(C) chromatin immunoprecipitation assay
(D) fluorescence in situ hybridization assay
Official Answer Key:
C
Step-by-Step Solution & Explanation
### **Official Solution & Step-by-Step Derivation (GATE BT 2012 Q.13)**
**1. Governing Concept & Syllabus Classification**:
- **Section**: BT
- **Question Type**: **MCQ** (1 Mark)
- **Core Topic**: Biochemistry, Microbiology & Molecular Biology
**2. Detailed Step-by-Step Analytical & Scientific Derivation**:
- **Systematic Conceptual & Option-by-Option Evaluation**:
- Problem Focus: *"Protein-DNA interactions in vivo can be studied by..."*
- **Option (C) [CORRECT]**: *"chromatin immunoprecipitation assay"*
- Validated by canonical biochemistry/biotechnology literature. Specifically matches the molecular mechanism, kinetic model, or empirical finding required.
- **Option (A) [INCORRECT]**: *"gel shift assay"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (B) [INCORRECT]**: *"Southern hybridization"* does not satisfy the precise boundary conditions or molecular specificity.
- **Option (D) [INCORRECT]**: *"fluorescence in situ hybridization assay"* does not satisfy the precise boundary conditions or molecular specificity.
**3. Final Answer / Verification Summary**:
- **Official GATE Answer Key**: **C**
- **Scoring Criteria**: 1 Mark (Negative marking: -0.33 marks).
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